Involvement of a putative CRP - like binding sequence and a CRP - like protein in 1 glucose - mediated catabolite repression of thn genes in Rhodococcus sp . strain
نویسندگان
چکیده
Involvement of a putative CRP-like binding sequence and a CRP-like protein in 1 glucose-mediated catabolite repression of thn genes in Rhodococcus sp. strain 2 TFB. 3 4 Laura Tomás-Gallardo, Eduardo Santero and Belén Floriano* 5 6 7 8 Centro Andaluz de Biología del Desarrollo, CSIC-Universidad Pablo de Olavide-Junta de 9 Andalucía, Seville, Spain. 10 11 12 13 14 15 *For correspondence: Belén Floriano. Centro Andaluz de Biología del Desarrollo, 16 Carretera de Utrera Km 1, 41013-Seville, Spain. Tel. (+34) 95 4349053; Fax (+34) 95 17 4349376. E-mail: [email protected] 18 19 20 21 Running title: CRP catabolite repression of thn genes in TFB 22 23 Copyright © 2012, American Society for Microbiology. All Rights Reserved. Appl. Environ. Microbiol. doi:10.1128/AEM.00700-12 AEM Accepts, published online ahead of print on 25 May 2012
منابع مشابه
Involvement of a putative cyclic amp receptor protein (CRP)-like binding sequence and a CRP-like protein in glucose-mediated catabolite repression of thn genes in Rhodococcus sp. strain TFB.
Glucose catabolite repression of tetralin catabolic genes in Rhodococcus sp. strain TFB was shown to be exerted by a protein homologous to transcriptional regulators of the cyclic AMP receptor (CRP)-FNR family. The protein was detected bound to putative CRP-like boxes localized at the promoters of the thnA1 and thnS genes.
متن کاملEffect of vfr mutation on global gene expression and catabolite repression control of Pseudomonas aeruginosa.
Vfr of Pseudomonas aeruginosa is 91% similar to the cAMP receptor protein (CRP) of Escherichia coli. Based on the high degree of sequence homology between the two proteins, the question arose whether Vfr had a global regulatory effect on gene expression for P. aeruginosa as CRP did for E. coli. This report provides two-dimensional polyacrylamide gel electrophoretic evidence that Vfr is a global...
متن کاملRole of the cAMP-Dependent Carbon Catabolite Repression in Capsular Polysaccharide Biosynthesis in Klebsiella pneumoniae
K. pneumoniae is the predominant pathogen isolated from liver abscesses of diabetic patients in Asian countries. Although elevated blood glucose levels cause various immune problems, its effects on K. pneumoniae virulence are unknown. This study investigated the regulation of capsular polysaccharide (CPS) biosynthesis, a major determinant for K. pneumoniae virulence, in response to exogenous gl...
متن کاملCatabolite repression of the propionate catabolic genes in Escherichia coli and Salmonella enterica: evidence for involvement of the cyclic AMP receptor protein.
Previous studies with Salmonella enterica serovar Typhimurium LT2 demonstrated that transcriptional activation of the prpBCDE operon requires the function of transcription factor PrpR, sigma-54, and IHF. In this study, we found that transcription from the prpBCDE and prpR promoters was down-regulated by the addition of glucose or glycerol, indicating that these genes may be regulated by the cyc...
متن کاملMolecular and biochemical characterization of the tetralin degradation pathway in Rhodococcus sp. strain TFB
The tetralin biodegradation pathway in Rhodococcus sp. strain TFB, a Gram-positive bacterium resistant to genetic manipulation, was characterized using a proteomic approach. Relative protein expression in cell free extracts from tetralin- and glucose-grown cells was compared using the 2D-DIGE technique. Identification of proteins specifically expressed in tetralin-grown cells was used to charac...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2012